Fig. 3

Clozapine did not alter Th cell subsets in the periphery. Tregs and Th17 cells were analyzed by flow cytometry 5 d.p.i (a) and 15 d.p.i (b; gating strategy shown in Additional file 3). Shown are the means and SEM of individual mice from one experiment with 5/group (a) or 2–3 experiments combined with 10–15 mice/group (b). c Representative dot plots of intracellular cytokine staining from spleen 15 d.p.i. d Graph of IFN-γ⁺ and IL-17A⁺ cells presented as percentage of CD4. Shown are means and SEM of individual mice from one experiment (n = 5 mice/group). e Splenocytes were re-stimulated with MOG peptide at 5 d.p.i for 72 h and IFN-γ and IL-17A in the supernatant measured by ELISA. Shown are means and SEM of individual mice from one experiment. Shown are the means and SEM of individual mice from one experiment (n = 5 mice/group). f Splenocytes were re-stimulated with MOG peptide 15 d.p.i for 72 h. IFN-γ and IL-17A in the supernatant measured by ELISA. Shown are the means and SEM of individual mice from an experiment representative of four (n = 5 mice/group). Statistical analyses were performed using two-way ANOVA and Sidak’s multiple comparison test