Fig. 5From: Mu-opioid receptor and delta-opioid receptor differentially regulate microglial inflammatory response to control proopiomelanocortin neuronal apoptosis in the hypothalamus: effects of neonatal alcoholIn vitro effects of ethanol with or without opioid receptor agonists on cytokine secretion from hypothalamic microglia in primary cultures. Showing the changes in the protein levels of pro-inflammatory cytokines TNF-α (a), IFN-γ (b), IL-1α (c), IL-1β (d), IL-6 (e), MIP-3α (f), MCP-1 (g), M-CSF (h), CXCL1 (i), and RANTES (j) and anti-inflammatory cytokines IL-4 (k) and IL-13 (l) in microglial conditioned medium following 24-h treatment with ethanol (50 mM) with or without MOR agonist (DAMGO, 50 μM) or DOR agonist (DPDPE, 10 nM). Data are represented as mean ± SEM (n = 6). Data were compared by one-way analysis of variance (ANOVA) and the Newman-Keuls posttest. Differences between control and other treatment groups or ethanol and ethanol and opioidergic drug groups are shown by lines with p values on the top of bar graphsBack to article page