Fig. 5
From: The involvement of mast cells in the irinotecan-induced enteric neurons loss and reactive gliosis
Mast cell pre-degranulation prevents CPT-11-induced increase of GFAP and S100β immunostaining and reduction of HuC/D immunostaining in duodenum of mice. a Representative images illustrating GFAP, S100β, and HuC/D immunostaining in the mucosa, submucosa, and myenteric plexuses. Scale bar corresponds to 50 μm (GFAP and S100β) or 20 μm (HuC/D). b Graphs represent the mean ± SEM of the percentage of GFAP, S100β, or HuC/D positive immunostaining in duodenum related to total tissue in five (GFAP and S100β) or ten (HuC/D) microscope field per mice from six mice in each group quantified using Photoshop. # p < 0.05 versus control group. *p < 0.05 versus CPT-11 group. One-way ANOVA followed by Bonferroni