Fig. 1

SMI treatment of monocytes inhibits CD40-induced trans-endothelial migration by limiting ROS production. Human monocytes were treated with either SMI 6877002 (1–10 μM) or vehicle for 1 h and stimulated with G28.5 (agonistic CD40 antibody) for 16 h, or pretreated with G28.5 for 1 h and then stimulated with SMI 6877002 for 16 h. a Monocyte trans-endothelial migration was studied in vitro using hCMEC/D3 cells by Transwell migration [31]. b ROS production measured as mean fluorescence intensity (MFI) of rhodamine 123. c CD40-induced monocyte migration in the presence or absence of the ROS scavenger luteolin (50 μM), measured in time-lapse migration. Experiments were performed in triplicate using buffy coats from 3 (1A), 4 (1B), or 3 (1C) human donors, and results are presented as the mean ± SEM. */#P < 0.05, **/##P < 0.01,***/###P < 0.001 as determined by Student’s t test. #luteolin vs control