Fig. 2

CD40-TRAF6-inhibiting SMI 6877002 improved the inflammatory phenotype of monocytes. Human monocytes were treated with SMI 6877002 (2.5 μM) for 1 h and stimulated with G28.5 (agonistic CD40 antibody) for 16 h. a CD14⁺ (classical), CD14⁺/CD16⁺ (intermediate), and CD16⁺ (non-classical) subsets of monocytes were measured by flow cytometry. b HLA-DR, CD80, and CD86 mean fluorescent intensity (MFI) measured in the classical monocyte subset (CD14⁺) by flow cytometry. c TNF, IL-6, and IL-10 production were measured by ELISA. Experiments were performed in triplicate using buffy coats of 2 (flow cytometry) or 4 (ELISA) human donors, and results are presented as the mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001 as determined by Student’s t test