Fig. 4

Prolonged SMI treatment does not affect EAE development in mice. Mice were treated with vehicle or 10 μmol/kg SMI 6877002 starting 3 days prior to EAE induction until 17 days after immunization. a Clinical scores of mice treated with vehicle or SMI 6877002. Experiments were performed with 14 animals in the vehicle- and SMI-treated groups and 6 control animals without EAE induction. b SMI 6877002 treatment reduces the percentage of Mac3⁺ cells in the cerebellum of EAE mice at the peak of disease (scale bar Mac3 staining 400 μm), but has no effect on T cell infiltration into the cerebellum (scale bar CD3 staining 200 μm). c Flow cytometric analysis demonstrates that SMI 6877002 treatment results in a shift in the CD4/CD8 T cell balance in lymph nodes. Analysis was performed in lymph nodes of six animals per group sacrificed at the peak of disease. Results are presented as the mean ± SEM. *P < 0.05, ***P < 0.001 as determined by Student’s t test