Fig. 3

Immunocytochemistry of GFP⁺LN⁻ cells in the presence of miR-101a inhibitor or mimic. a Cultures were stained with antibodies against Iba1 and CD11b. b Cultures were stained with antibodies against TREM-2 and CX3CR1. miR-101a inhibitor significantly decreased the number of Iba1⁺TREM-2⁺CX3CR1⁺ small, round cells, while miR-101a increased the number of these. Bar = 50 μm. c–f The numbers of cells positive for c Iba1, d CD11b, e TREM-2, and f CX3CR1 (n = 5). Data are presented as means ± S.D. *P < 0.05, **P < 0.01 by Mann-Whitney test