Fig. 3
From: Evidence for an early innate immune response in the motor cortex of ALS
MCP1+ and CCR2+ cells are visualized in motor cortex and spinal cord of MCP1-CCR2-hSOD1G93A mice. a, b Representative images of MCP1+ and CCR2+ cells with no immunohistochemistry enhancement for mRFP or eGFP. Very few cells are present in the motor cortex of MCP1-CCR2-WT mice. Insets enlarged to the right (a’, b’). c–h Representative images of MCP1+ and CCR2+ cells with no immunohistochemistry enhancement for mRFP or eGFP. Number of cells are present vary at different stages of disease initiation and progression throughout the motor cortex in MCP1-CCR2-hSOD1G93A mice. Insets enlarged to the right (c’–h”). i Representative images of MCP1+ and CCR2+ cells with no immunohistochemistry enhancement for mRFP or eGFP. Cells are noticeable around the meninges surrounding the cingulate cortex in MCP1-CCR2-hSOD1G93A mice. Insets enlarged to the right (j–n). o Representative images of MCP1+ and CCR2+ cells with no immunohistochemistry enhancement for mRFP or eGFP. Few cells are present in the ventral horn of the MCP1-CCR2-WT mice spinal cord. Inset enlarged to the right (o’). p Representative images of MCP1+ and CCR2+ cells with no immunohistochemistry enhancement for mRFP or eGFP. Numerous cells are present in MCP1-CCR2-hSOD1G93A mice at the end stage. Insets enlarged to the right (p’–p”’). MCP1+ and CCR2+ cells do not co-localize with neuronal marker (Map2) in the motor cortex or spinal cord