Fig. 7

Sac-1004 inhibits glial activation after I/R. Immunofluorescence staining (a, b) of CD11b and GFAP in the ischemic cortex of the sham, vehicle-ischemia, and Sac-1004-ischemia groups 3 h after I/R. Profound expression of CD11b and GFAP was observed in the vehicle-ischemia group compared with that in the sham group, whereas the Sac-1004-ischemia group showed reduced expression of CD11b and GFAP. Scale bar = 50 μm. Glial activation(c, d) is quantified by the intensity of CD11b and GFAP immunofluorescence (n = 5 per group; ^** P < 0.01 and ^*** P < 0.001 vs sham group, ^## P < 0.01 and ^### P < 0.001 vs vehicle-ischemia group). The bars indicate the means ± SEM