Fig. 1

Immunofluorescence staining of central nervous tissues. Cryosections of rat hippocampus (A) and cerebellum (B) as well as primate cerebellum (C) were incubated with patient serum (A–C) or with control serum (A′–C′) (A–C1, A′–C′ 1:100, A–C2, C3 1:50) in the first step and with Alexa Fluor 488-labeled goat anti-human IgG in the second step (green). Nuclei were counterstained by incubation with TO-PRO-3 iodide (blue). A fine-granular staining of the stratum moleculare (sm) was obtained. On the hippocampus, the sm internum was stained more intensely than the sm externum. Scale bar: 100 μm (A–C1, A′–C′), 20 μm (A–C2, C3). h hilus, sm stratum moleculare, smi stratum moleculare internum, sme stratum moleculare externum, sg stratum granulosum, sp stratum purkinjense