Fig. 7
From: The role of microglial P2X7: modulation of cell death and cytokine release
Secretome of microglia treated with P2X7 antagonist upon pro-inflammatory stimulation. Microglia were incubated with 1 μM P2X7 antagonist A-804598 for an hour before exposure to 100 ng/ml LPS or 100 ng/ml LPS plus 10 ng/ml IFNγ for 22 h in the presence or absence of 380 μM BzATP for additional 2 h. a Heat map and hierarchical clustering of detectable 30 proteins released from microglia measured by Luminex multiplex. b–e Histogram of IL1β (b), IL1α (c), TNFα (d), and IL6 (e) release obtained from a. Data are shown as mean + SD of triplicates. Experiment repeated twice independently. One-way ANOVA followed by Tukey’s post hoc test. ****P < 0.0001