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Fig. 6 | Journal of Neuroinflammation

Fig. 6

From: The Carbon monoxide releasing molecule ALF-186 mediates anti-inflammatory and neuroprotective effects via the soluble guanylate cyclase ß1 in rats’ retinal ganglion cells after ischemia and reperfusion injury

Fig. 6

Effect of ALF-186 treatment on retinal protein and mRNA expression of IL-6 in tissue and IL-6 serum levels. a Fold induction of IL-6 mRNA expression in ischemic retinal tissue compared to GAPDH in relation to the corresponding non-ischemic retinae analyzed by RT-PCR (n = 8; data are mean ± SD; IRI vs. IRI + ALF-186, *** = p < 0.001, IRI + ALF-186 vs. IRI + ODQ + ALF-186, ** = p < 0.01). b, c Representative Western Blot image and densitometric analysis of n = 8 Western Blots showing the suppression of retinal IL-6 protein compared to β-Actin after ALF-186 (data are mean ± SD; IRI vs. IRI + ALF-186, * = p < 0.05). d Histological images, showing that IL-6 protein expression is increased due to IRI + PBS, while ALF-186 treatment attenuates this effect. Quantification via frequency density of IL-6 protein expression in both groups by histogram analysis revealed a significant downregulation of IL-6 due to ALF-186 treatment (p < 0.05). e IL-6 serum levels in peripheral blood serum after ALF-186 treatment (data are mean ± SD; n = 8; Sham vs. IRI, *** = p < 0.001; IRI vs. IRI + ALF-186, ** = p < 0.01 and IRI + ALF-186 vs. ODQ + IRI + ALF186, * = p < 0.05)

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Journal of Neuroinflammation

ISSN: 1742-2094