Fig. 1

Brain microvessel endothelial cells (BMEC) respond to IL-1β in a MyD88-dependent manner. a PE-CAM (green) and p65 NF-kB (red) immunofluorescence in WT and MyD88KO BMEC that were treated with PBS or IL-1β (50 ng/mL) for 30 min. Blue = DAPI. Arrows indicate examples of PE-CAM-positive cells with nuclear concentration of NF-κB. Scale bar = 50 μm. b Percentage of BMEC with nuclear concentration of NF-κB. c Inflammatory gene expression in WT and MyD88KO BMEC that were treated with PBS or IL-1β (50 ng/mL) for 4 h. n = 2 to n = 3 per group. Data are expressed as mean ± SEM. ***p < 0.001 vs. WT PBS group, #p < 0.05 vs. WT IL-1β group