Table 1 Primer/Probe used for qRT-PCR
Primer/probe set | Â | Â |
|---|---|---|
Hmbs | Forward Primer | 5'-TCC CTG AAG GAT GTG CCT AC-3' |
| Â | Reverse Primer | 5'-ACA AGG GTT TTC CCG TTT G-3' |
|  | Probe | Universal Probe Library: Probe 79—Roche |
Hprt1 | Forward Primer | 5'-TGA TAG ATC CAT TCC TAT GAC TGT AGA-3' |
| Â | Reverse Primer | 5'- AAG ACA TTC TTT CCA GTT AAA GTT GAG-3' |
|  | Probe | Universal Probe Library: Probe 22—Roche |
Iba1 | Forward Primer | 5'-GGATTTGCAGGGAGGAAAA -3' |
| Â | Reverse Primer | 5'- TGGGATCATCGAGGAATTG-3' |
|  | Probe | Universal Probe Library: Probe 3—Roche |
Gfap | Forward Primer | 5'- TGGAGGAGGAGATCCAGTTC-3' |
| Â | Reverse Primer | 5'- AGCTGCTCCCGGAGTTCT-3' |
|  | Probe | Universal Probe Library: Probe 69—Roche |
TNFα | Forward Primer | 5'- CTGTAGCCCACGTCGTAGC-3' |
| Â | Reverse Primer | 5'- TTGAGATCCATGCCGTTG-3' |
|  | Probe | Universal Probe Library: Probe 25—Roche |
IL-1β | Forward Primer | 5'- AGTTGACGGACCCCAAAAG-3' |
| Â | Reverse Primer | 5'- AGCTGGATGCTCTCATCAGG-3' |
|  | Probe | Universal Probe Library: Probe 38—Roche |
IL6 | Forward Primer | 5'- GCTACCAAACTGGATATAATCAGGA-3' |
| Â | Reverse Primer | 5'- CCAGGTAGCTATGGTACTCCAGAA-3' |
|  | Probe | Universal Probe Library: Probe 6—Roche |
KC/GRO | Forward Primer | 5'- ACTCCAACACAGCACCATGA-3' |
| Â | Reverse Primer | 5'- TGGTCTGCAGGCACTGAC-3' |
|  | Probe | Universal Probe Library: Probe 49—Roche |