Fig. 8

NAM administration recovers the survival and function of puberty-born neurons. A BrdU/NeuN assay shows the survival of puberty-born neurons in the DG until PND65; BrdU/NeuN/c-fos assay shows the active puberty-born neurons. NeuN (green pixels), BrdU (red pixels), c-fos (pink pixels), and DAPI (blue pixels). B The percentage of puberty-born neurons in DG neurons. C The percentage of active puberty-born neurons in BrdU/NeuN cells. Data are presented as mean ± SEM for each group (n = 4 per group). n.s. not significant; *p < 0.05, and **p < 0.01