Fig. 6

Inhibiting ferroptosis attenuated I/R injury and RGC loss. A Representative H&E-stained images among groups. B Bar plots showing the mean thickness of IPL among groups (n = 9/group). C Immunofluorescence image labeling RGCs in retina with Tuj-1 (green) and Brn3a (red), and the merge image of two channels were shown above (scale bar = 50 μm). D Bar plots showing the RGC survival rate among groups (n = 5/group). The mRNA expression levels of Ptgs2 (E), Slc7a11 (F), and Gpx4 (G) in retina cells were measured with real-time quantitative PCR. Retina lysates were collected and the proteins were subjected to western blot analysis to detect the level of GPX4 (β-actin was used as a control) (H, I). J Flow cytometry histogram showing the expression of ROS in retinal tissue. K MFI graph showing the expression of ROS in retinal tissue (n = 3/group). L Level of 4-HNE in retina tissue of among groups (n = 4/group)