Fig. 7

Inhibiting ferroptosis inhibited inflammatory response and cell activation in vivo and vitro. The mRNA expression levels of Il1b (A), Il6 (B), Tnfa (C), Ccl3 (D) and Ccl4 (E) in retina cells were measured with real-time quantitative PCR. F Immunofluorescence image labeling activated microglia with Iba1(red) is shown. Cell nucleuses were stained with DAPI (blue). G Statistical analysis of the proportion of IBA1+ cells among groups (n = 5/group). H Flow cytometry histogram (left) and MFI graph (right) showing the expression of ROS in retinal IBA1+ cells (n = 5/group). I Flow cytometry histogram showing the expression of CD11b+ cells in retinal tissue. J Statistical analysis of the proportion of CD11b+ microglia among groups (n = 3/group). K Primary retinal microglia lysates were collected and the proteins were subjected to western blot analysis to detect the level of GPX4 (β-actin was used as a control) among groups (n = 3/group). L Flow cytometry histogram (left) and MFI graph (right) showing the expression of ROS in purified microglia (n = 5/group). The mRNA expression levels of Il1b (M), Il6 (N), Tnfa (O), Ccl3 (P) and Ccl4 (Q) in microglia were measured with real-time quantitative PCR