Fig. 2

GPR43 expression is reduced in IEL upon EAE. A Levels of transcripts encoding GPR41, GPR43 and GPR109a were determined by RTqPCR and normalised with the levels of gapdh on CD19⁺ B-cells, CD4⁺ TCRαβ⁺ and CD8αβ⁺ TCRαβ⁺ T-cells isolated from spleen of healthy mice. Values represent mean ± SEM from 9 mice per group. ND: not detected. (B-D) GPR43 expression at protein level was determined on B CD19⁺ B cells, TCRγδ⁺ and TCRαβ⁺ T-cells and C on CD4⁺, CD8αβ⁺ and CD8αα⁺ subsets of TCRαβ⁺ T-cells from spleen (SPL), intraepithelial lymphocytes (IEL) and lamina propria lymphocytes (LPL) obtained from healthy (black) or EAE mice at 15dpi (red). Values are the mean fluorescence intensity (MFI) associated to GPR43 immunostaining. Data were obtained from 6–8 mice per group. Each symbol represents data obtained from an individual mouse. Mean ± SD are indicated. D Representative histogram of GPR43 expression analysed by flow cytometry. Control (filled grey), healthy mice (black) and EAE mice (red). *, p < 0.05; **, p < 0.01; by one-way ANOVA followed by Tukey’s post-hoc test (A) or unpaired Student’s t-test (B and C)