Fig. 2
From: Smad7 in the hippocampus contributes to memory impairment in aged mice after anesthesia and surgery
Unilateral nephrectomy under anesthesia induced upregulation of Smad7 expression in neurons of the hippocampal CA1 region. A Expression of Smad7 at the mRNA level in the hippocampus was measured by qRT-PCR assay at day 3 after surgery. The data are presented as the mean ± standard error (n = 7). ****P < 0.0001. B Representative western blots of Smad7 in the hippocampus at day 3 after surgery. C Expression of Smad7 protein in the hippocampus was determined by western blotting assay on day 3 after unilateral nephrectomy. The data are presented as the mean ± standard error (n = 6). **P < 0.01. D, E. Representative confocal images of Smad7 expression in neurons (NeuN) and microglia (Iba1) in the (D) CA1 region and (E) CA2/3 region of the hippocampus