Fig. 5
Validation of the MS-generated histone Kla sites. A, B Immunoblotting results showing the changes in levels of H3K14, H3k18, H4K8 and H4K12 Kla sites in the indicated BV2 (A) or HMC3 (B) cells using their corresponding site-specific Kla antibodies. C Immunoblotting evaluation of changes in Kla sites of H3K14, H3k18, H4K8 and H4K12 in hippocampus tissues from young and naturally aged mice with the corresponding site-specific Kla antibodies (n = 3 mice per group). D, E Immunoblotting results illustrating changes in Kla of H3K18, pan-Kla in hippocampus tissues of WT, and FAD4T and app/ps1 C57 mice (n = 3 mice per group in figure D, n = 5 WT mice and 4 app/ps1 mice in figure E. F–H Immunoblotting displaying changes in H3K18, pan-Kla in cortex of naturally aged C57 mice, FAD4T, app/ps1 C57 mice and corresponding counterparts (n = 5 WT mice and 4 app/ps1 mice in figure F, n = 4 mice per group in figure G, H). Each bar represents the mean ± s.d. for triplicate experiments, *p < 0.05, **p < 0.01, ***p < 0.001, N.S.: no significance