Fig. 1

Mast cells in human infarct region of samples obtained by acute, sub-acute and chronic (late) timepoints: MCs analyzed and quantified by Toluidine Blue (TB) staining in human brain samples. A Acute, sub-acute and later sub-acute (old) stages, with MC marked with red arrows. Mast cells (histamine filled) stains in purple (TB staining). Magnification; 20X. B Quantification of MCs shows an increase in MC infiltration during the acute phase and significant increase of MCs at the sub-acute phase. At this phase, the histamine-filled MCs are higher compared to emptied MCs. The late sub-acute phase shows an increase in the empty MCs. Data are expressed as mean ± SEM, as well as individual values. Two-way ANOVA and Turkey’s multiple comparison show the statistical significance among the groups (n = 4 per group). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001