Fig. 3

Flow cytometry analysis on the brain tissue revealed that there is a significant increase of MC infiltration in the brain of post-stroke mice compare to sham mice. Importantly, administration of cromolyn orally as early as 3 h, 10 h and 24 h caused a decrease in MC numbers in the brain post-stroke compared to the vehicle treatment groups at 24 h and 3 days after stroke. Two-way ANOVA and Turkey’s multiple comparison show the statistical significance among the groups. Data are expressed as mean ± SEM, as well as individual values, and are obtained from three independent experiments at different time points (n = 4–6). *P < 0.05, **P < 0.01, ***P < 0.001.