Fig. 1

A Overview of differentiation timeline for iPSC-derived NPCs showing representative brightfield images on the last day of each stage. Representative immunofluorescence images of iPSC-derived NPCs from healthy control lines and familial AD lines harbouring a PSEN2 (N141I) mutation. The cells were stained for B the neural progenitor markers Pax-6 (red) and Nestin (green), C a pluripotency marker Oct3 (green) and all nuclei were counterstained with DAPI (blue). Insert shows higher magnification. All scale bars = 50 μm. The percentage of iPSC-derived NPCs positive for D Pax6 and E nestin, with each bar displaying the mean ± SD of three cell lines with n ≥ 2 independent experiments per line. Immunofluorescence staining of all iPSC and astrocyte cell lines can be found in Additional file 1: Fig. S2, S3 and S4