Fig. 5
From: iPSC-derived PSEN2 (N141I) astrocytes and microglia exhibit a primed inflammatory phenotype
A An example of the processing of an immunofluorescence image of iPSC-derived microglia-like cells stained for IBA1 (red), the result of image processing is shown in binary and was followed by skeletonisation (green). An overlay of the original IBA1 image (red) and skeleton (green) is shown for comparison. Automated analysis of the mean number of B junctions, C branches, D endpoints and E longest branch length per cell. An unpaired t-test was used to test whether there were statistically significant differences between the means of AD-derived and healthy control microglia-like cells. The figure displays the mean ± SD of three cell lines with n = 2 independent experiments per line (*p < 0.05)