Fig. 6

Concentration of IL-6 secreted from healthy control or AD-derived astrocytes A basally and after exposure to 10 or 50 μg/mL LPS for 24 h. B Data from part A represented as individual cell lines. Cell lines were re-categorised based on their APOE ε3/ε3 (Ctrl-06, Ctrl-71 and fAD-08) or APOE ε3/ε4 (Ctrl-88, fAD-948 and fAD-950) genotype and shows the concentration of IL-6 secretion C basally and after exposure to 10 or 50 μg/mL LPS for 24 h. A two-way ANOVA with multiple comparisons and post hoc Dunnett’s test was used to test whether there were statistically significant differences between the means of AD-derived and healthy control astrocytes or APOE ε3/ε3 and APOE ε3/ε4 astrocytes (***p < 0.001). Statistical tests were not conducted for figure B. Figures A and C display the mean ± SD of three cell lines with n ≥ 2 independent experiments per line, B display the mean ± SD of n ≥ 2 independent experiments per line