Fig. 3
Single-dose intranasal IFNα treatment stimulates ISG expression in OSNs and OB. A Volcano plot of DEGs within immature OSNs following intranasal saline (negative) or IFNα (positive) treatment (1 × 104 U, 12 h, N = 539 saline, 561 IFNα from two mice). B Volcano plot of DEGs within mature OSNs following intranasal saline (negative) or IFNα (positive) treatment (1 × 104 U, 12 h, N = 1816 saline, 2076 IFNα from two mice). C Representative ISG expression within nasal cavity and olfactory bulb homogenates following intranasal IFNα (8 × 104 U, 24 h, N = 5–6) treatment. D FISH of Ldlrad3 expression (red) following vehicle or IFNα co-administration during intranasal VEEV–ZPC (10 pfu, 12 hpi, N = 3). Error bars indicate mean ± SEM. ΔCq were compared via unpaired t test. Ldlrad3 expression was compared by one-way ANOVA, followed by Tukey multiple comparison. Statistical values are indicated as follows *P < 0.05; **P < 0.01; ***P < 0.001, ****P < 0.0001 unless otherwise stated