Fig. 3

Depletion of microfold cells enhanced microglial phagocytosis in 5xFAD mice. A Images of Aβ plaques (thioflavin S, green) and microglia (Iba-1, red); scale bar, 20 μm. B The quantification of microglia surrounding Aβ plaques (n = 9 per group). C Immunofluorescence images of lysosome (CD68, green), microglia (Iba-1, red), and Aβ (6E10, blue); scale bar, 20 μm. D, E The quantification of phagosome (CD68⁺Iba-1⁺/Iba-1⁺) area (%) D and internalized Aβ (Iba-1⁺6E10⁺/6E10⁺) area (%) E (n = 9 per group). F–I Flow cytometric analysis of the quantification of and CD68-positive microglia (CD45^intCD11b⁺) F and G and MeX04-positive microglia (CD45^intCD11b⁺) H and I in the brain (n = 8 per group). Bars represent as mean ± standard deviation. Statistical analysis included the Student’s t-test. *P < 0.05 vs. 5xFAD mice