Fig. 5
Prominent Langerhans and mast cell accumulation in CRPS affected finger of acute ipsilateral CRPS skin. Skin sections from finger biopsies were immunofluorescently labelled. A-C Representative images and quantification of Langerhans cells (S100+CD207+) in the epidermis are depicted. Dashed lines indicate the epidermal-dermal border (nHC = 24\1, nacute = 19, nchronic = 6). Scale bar = 50 µm. D-F Representative images and quantification of mast cells (tryptase+) in the dermis but also close to the epidermal-dermal border (dashed lines). Nuclei were stained with Hoechst33342. Mast cells were quantified per area of dermis (nHC = 24, nacute = 18, nchronic = 6). Scale bar = 100 µm. G, H Pearson correlation of Langerhans and mast cell densities or mast cell densities and mean numeric pain scale (NRS). Dashed lines indicate thresholds determined from data of HC group. I Tryptase concentrations were determined in plasma from patients with acute and chronic CRPS and compared with HCs (nHC = 25, nacute = 16\1, nchronic = 6). Data are shown as mean ± SD; Welch’s ANOVA and Dunnett’s tests; *: p < 0.05; **: p < 0.01. CL: contralateral; HC: healthy controls; IL: ipsilateral