Fig. 6

Rapamycin inhibits NLRP1 inflammasome signaling and exhibits a neuroprotective effect in depressive-like mice. A–F Representative immunoreactive bands and statistical results show that rapamycin inhibited CSDS-induced increase in the expression of hippocampal NLRP1 (A), ASC (B), Caspase1 (C), IL-6 (D), IL-1β (E), TNF-α (F). n = 6. G–I Representative immunoreactive bands and statistical results show that rapamycin restored the levels of hippocampal BDNF (G), p-TrkB (H), Bcl-2/Bax (I) and cleaved caspase-3 (J) in depressive-like mice. n = 6. K–N Representative images (K) and statistical results (L–N) show that rapamycin prevented CSDS-induced decrease in the number of NeuN in hippocampal CA1, CA2, CA3 region. n = 3, scale bar = 50 μm. Data expressed as mean ± SEM, *p < 0.05, **p < 0.01 vs. control group or CSDS group