Fig. 2

Macrophage accumulation in the lungs is affected by α7nAChR signaling. A WT and α7nAChR^−/− mice were injected with a sublethal dose of LPS. After 48h lungs were removed, digested, and analyzed using flow cytometry. CD11b-positive cells were selected and tested with antibodies against Ly6-G and F4/80 to identify neutrophils and macrophages, respectively. Results were analyzed and calculated using FACSDiva software and GraphPad Prism. B Plots representing the number of WT and α7nAChR^−/− macrophages (top right, n = 5/group), neutrophils (bottom left, n = 4/group), and resident macrophages (bottom right, n = 5/group) in digested lungs. Residents were identified as CD11b-F4/80 + Siglec F + . Body temperature at 48h is shown at top right (n = 7/group). C WT mice were injected with either a sublethal dose of LPS or 3mg/kg PNU-282987 followed by LPS 15 min later. The dose of LPS used was higher than for part A to generate more severe conditions for WT mice. After 48h lungs were removed, digested and analyzed using flow cytometry. CD11b positive cells and residents were selected and analyzed as above. Results were analyzed with FACSDiva software and calculated with GraphPad Prism. D Plots depicting the number of macrophages (top right, n = 7/group), neutrophils (bottom left, n = 7/group), and resident macrophages (bottom right, n = 6/group). Body temperature at 48h is shown at top left (n = 7/group). Statistical analysis was performed using a paired t test