Fig. 4

Effect of α7nAChR deficiency on migration does not depend on other cell types. A Representative dot plots of flow cytometry showing migrated red (WT) and green (α7nAChR^−/−) monocytes in male α7nAChR^−/− recipient mice. The leukocyte distribution in lungs before the adoptive transfer (Upper panel) and at 48 h after adoptive transfer and LPS administration (lower panel) are presented. B Bar graphs representing the amount of WT and α7nAChR-deficient macrophages detected in organs by flow cytometry. The experimental setup is same, as depicted in Fig. 3A, using 8-week-old male and female α7nAChR^−/− recipients instead of WT (n = 4). Statistical analysis was performed using a student’s t test. **P < 0.01