Fig. 1

Establishment of the EAMG rat model. A Clinical scores and B body weight of 20 rats were assessed to evaluate the establishment of the rat model. Arrows and colors indicate the timing of immunizations on day 0 and day 30. Significant differences in clinical scores were observed on day 40, while significant differences in average weights were observed on day 42 using Sidak’s correction. C α-BTX staining and its fluorescence intensity (normalized to CFA) at a magnification of 100 μm. D The relative intensity in the CFA group is significantly higher than in the EAMG group. E The level of AChR-specific antibodies in serum was significantly higher in the EAMG group compared to the CFA group. Statistical analysis was performed using repeated measures ANOVA and t test, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, ns = no significant difference