Fig. 3

Visualization of differentially expressed genes (DEGs) in eight pathways. A Two-dimensional t-SNE plot showing the separation between CFA and EAMG rats, with each color representing a different sample group. B–E The top enriched pathways with the highest NES were plotted in each subplot for sequenced proteins in the Kyoto Encyclopedia of Genes and Genomes (KEGG, B) and the biological process (BP, C), molecular function (MF; D), and cellular component (CC, E) components in the GO database, respectively. The CNS-related pathways are highlighted in dark crimson, and one pathway associated with the epithelium is highlighted in dark cyan. F–G Two heatmaps illustrate the levels of differentially expressed genes within eight pathways. In each heatmap, the left panel shows the log-transformed p values obtained by the t tests, while the central panel shows the relative expression value normalized using the z scores