Fig. 5

The impact of dopamine incubation in the periphery on the bEnd.3 in vitro was investigated. A The dopamine level in serum was significantly higher in EAMG rats, as measured by ELISA. B The proliferative effects of DA on the bEnd.3 cell lines by CCK-8. C The expression of ZO1, CD31, Occludin, Claudin5 and CXC43 proteins in bEnd.3 cell lines was compared after incubation by western blot. D The dopamine-treated bEnd.3 cell lines exhibited significantly lower levels of ZO1, CD31, and Claudin5 expression. E Among the tested gene sets in serum (Tjp1, Tjp2, Ocln, Cldn5, Pecam1, and Gja1), dopamine-treated cell lines exhibited significantly reduced levels of dopamine, except for Ocln and Gja1 using qPCR. Statistical analysis was performed using one-way ANOVA and t tests, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, ns = no significant difference