Fig. 2

Hamp^GFAP cKO mice had hippocampal atrophy and decreased number of neurons in DG. A, B The weights of total brain, cerebellum, unilateral cortex, hippocampus and striatum were measured in 3-month-old (A) and 6-month-old (B) Hamp^GFAP cKO mice with their respective control mice (n = 6 and n = 7 per group in 3- and 6-month-old mice, respectively). C The weights of brain and hippocampus in 14-day-old Hamp^GFAP cKO and control mice (n = 7 and 5, respectively). D The brain weight of the E18.5-day-old Hamp^GFAP cKO and control mice (n = 3 per group). E Representative images of immunofluorescence staining of NeuN⁺ cells in 14-day-old Hamp^GFAP cKO and control mice. Scale bar: 200 µm. F, G Quantifications of the number of NeuN⁺ cells in hippocampus (F) and in DG region (G) of the Hamp^GFAP cKO and control mice (n = 7 and 5, respectively). H, I Immunofluorescence staining of NeuN⁺ cells (H) and the quantification (I) in hippocampal DG of 3-month-old Hamp^GFAP cKO and control mice (scale bar: 50 μm, n = 5 per group). Data were expressed as mean ± SEM. *p < 0.05, **p < 0.01 and ***p < 0.001