Fig. 3

Hamp^GFAP cKO mice exhibited reduced proliferation levels of hippocampal NSCs. A–D Immunofluorescence staining images and quantifications of Pax6⁺ cells (A, B) and Ki67⁺ cells (C, D) in the DG region of 14-day-old Hamp^GFAP cKO and control mice (scale bar: 50 μm, n = 5 per group). E–H Immunofluorescence staining images and quantifications of Pax6⁺ cells (E, F) and Ki67⁺ cells (G, H) in the DG region of E18.5-day Hamp^GFAP cKO and control mice (scale bar: 50 μm, n = 3 per group). I, J Immunofluorescence staining images of BrdU⁺ cells (I) and quantification (J) in the hippocampal DG of 3-month-old Hamp^GFAP cKO and control mice (scale bar: 50 μm, n = 4 per group). K–N Immunohistochemical staining images of DCX⁺ cells and quantification in the hippocampal DG of 14-day-old Hamp^GFAP cKO and control mice (K and L, n = 5 per group) and 3-month-old Hamp^GFAP cKO and control mice (M and N, n = 3 per group). Data were expressed as mean ± SEM. *p < 0.05, and ***p < 0.001