Fig. 5

Iron overload or Hamp knockdown in the hippocampus of WT mice induced TNF-α secretion and neurogenesis impairment. A Schematic diagram showing the injection groups of mice, and time points of treatment and analysis. B, C Immunohistochemical staining of DCX⁺ cells (B) and quantification (C) in the hippocampal DG of the control and FAC-treated mice (n = 4 per group). D, E. qRT-PCR detection of the mRNA levels of TNF-α (D) and BDNF (E) in the hippocampus of control and FAC-treated mice (n = 4 per group). F, G Immunohistochemical staining of DCX⁺ cells (F) and quantification (G) in the hippocampal DG of the control and Hamp KD mice (n = 5 per group). H, I Immunofluorescence staining images (H) and quantification (I) of Ki67⁺ cells in the DG region of the control and Hamp KD mice (n = 5 per group). J, L qRT-PCR detection of the mRNA levels of Hamp (J), TNF-α (K), and BDNF (L) in the hippocampus of the control and Hamp KD mice (n = 5 per group). Data were expressed as mean ± SEM. *p < 0.05, ***p < 0.001