Fig. 6

Inhibition of P2RX7 involves the MAPK Signaling Pathway. A Enrichment analysis was performed utilizing differentially expressed transcription factors and surface proteins derived from the DEGs extracted from seven distinct cellular subgroups (Excitatory neurons were split into Low-Ferro (Low-FS-Excit) and High-Ferro (High-FS-Excit) categories based on the Ferroptosis scores) in GSE143560. The bar chart shows the significantly enriched GO, KEGG, and WikiPathway terms. B Enrichment analysis was performed utilizing the DEGs extracted from seven distinct cellular subgroups. Notably, the MAPK signaling pathway emerged as the most significant pathway in the High-FS-Excit group (both in panels A and B). C A schematic diagram of transcriptome sequencing (these originate data from our murine model hippocampus tissues). D, E KEGG pathway enrichment analysis for DEGs in the KA (D) and Fer-1 (E) groups. The MAPK signaling pathway was the most significantly enriched in the KA (D) and Fer-1 (E) groups. F, G Western blots and quantification of the protein levels of P-ERK and ERK in the control, KA, sh-P2RX7 + KA and sh-Con + KA groups. H, I Western blots and quantification of the protein levels of P-P38 and P38 in the control, KA, sh-P2RX7 + KA and sh-Con + KA groups. J, K Western blots and quantification for comparing the protein levels of P-JNK and JNK in control, KA, sh-P2RX7 + KA and sh-Con + KA groups. The ratios of P-ERK/ERK, P-P38/P38 and P-JNK/JNK were increased in the KA group compared to control group, but decreased in the sh-P2RX7 + KA group compared to the sh-Con + KA group