Fig. 3

C-Mito^HF exacerbated endothelial cGAS activation in the SFO of HF mice. A Double-immunofluorescent staining of cGAS and endothelial marker CD31 in the SFO of mice. Scale bar = 50 μm. B Pearson coefficient analysis showed cGAS was mainly expressed in ECs of the SFO. C Relative fluorescent intensity analysis of cGAS showed that C-Mito^HF significantly exacerbated the increase of endothelial cGAS in the SFO of HF mice compared with C-Mito^Ctrl. D Immunoblotting of cGAS to detect its expression in the SFO. E Analysis showed that C-Mito^HF significantly exacerbated the increase of endothelial cGAS in the SFO of HF mice compared with C-Mito^Ctrl. F cGAMP level was measured in the SFO of mice. n = 8, P < 0.05, ANOVA LSD test