Fig. 5

C-Mito^HF highly expressed DHODH, which interacts with cGAS to facilitate brain endothelial cGAS activation. A Immunoblotting was conducted to detect DHODH expression in C-Mito^Ctrl and C-Mito^HF. B The results of immunoblotting showed that the expression of DHODH was higher in C-Mito^HF compared to C-Mito^Ctrl. t test. C Co-localization of DHODH and cGAS was detected by double-immunofluorescent staining in bEnd.3 cells. Scale bar = 10 μm. D The direct interaction between DHODH and cGAS was detected by co-immunoprecipitation. E, F Immunuoblotting results showed that C-Mito^HF significantly increased endothelial cGAS expression, while teriflunomide pre-treatment on C-Mito^HF significantly mitigated the cGAS-inducing effect of C-Mito^HF in bEnd.3 cells. G Teriflunomide pre-treatment on C-Mito^HF significantly mitigated the cGAMP-producing effect of C-Mito^HF in bEnd.3 cells. H Pre-treated with Teriflunomide on C-Mito^HF ameliorated the pro-inflammatory effect of C-Mito^HF in bEnd.3 cells. n = 6, P < 0.05, ANOVA LSD test