Fig. 7

C-Mito^HF promoted glial activation and neuronal sensitization in the SFO depended on endothelial cGAS in HF mice. A, B Double-immunofluorescent staining showed that compared with C-Mito^Ctrl, C-Mito^HF exacerbated microglial M1 polarization in the SFO of HF mice, which could be mitigated by endothelial cGAS KD in the SFO. Scale bar = 100 μm. C, D Double-immunofluorescent staining showed that compared with C-Mito^Ctrl, C-Mito^HF exacerbated astroglial A1 transition in the SFO of HF mice, which could be mitigated by endothelial cGAS KD in the SFO. Scale bar = 100 μm. E, F Double-immunofluorescent staining showed that compared with C-Mito^Ctrl, C-Mito^HF exacerbated neuronal sensitization in the SFO of HF mice, which could be mitigated by endothelial cGAS KD in the SFO. Scale bar = 100 μm. n = 8, P < 0.05, ANOVA LSD test