Fig. 6

Gene expression findings from the ipsilateral cortex suggesting interactive effects of T.gondii and CCI at 7-days and 18-weeks post-injury in male and female mice. Relative to T. gondii and/or CCI in isolation, male mice with combined T. gondii and CCI had evidence of altered gene expression related to neuroinflammation (A, B, D, F) and oxidative stress (C, E) at 7-days post-CCI, as well as ICAM1 at 18-weeks post-injury (G). In females, there was a significant T. Gondii * CCI interaction indicating that all groups had elevated gene expression of CCL4 when compared to Vehicle + Sham mice at 7-days post-injury (H), and the T. gondii + CCI group had decreased expression of MAP2 than all other groups at 18-weeks post-injury (I). G–I were analyzed with 2-way ANOVA and the significant T. Gondii * CCI interaction was further analyzed with Bonferroni post-hoc comparisons. These normally distributed data are displayed as mean ± SD. Data in A–F were not normally distributed and were therefore analyzed with Kruskal–Wallis tests and presented as median with interquartile range. *p < 0.05, **p ≤ 0.01. ***p ≤ 0.001