A pre-existing Toxoplasma gondii infection exacerbates the pathophysiological response and extent of brain damage after traumatic brain injury in mice

Table 5 Results of gene expression analyses from the ipsilateral cortex at 18-weeks post-CCI in male and female mice

Genes	Male					Female
	1. Vehicle + Sham (n = 5)	2. T. gondii + Sham (n = 5)	3. Vehicle + CCI (n = 5)	4. T. gondii + CCI (n = 4)	Significance	1. Vehicle + Sham (n = 5)	2. T. gondii + Sham (n = 4)	3. Vehicle + CCI (n = 5)	4. T. gondii + CCI (n = 5)	Significance
	Mean ± SD or Median [25,75 percentile]				Significance	Mean ± SD or Median [25,75 percentile]				Significance
BBB integrity/vascular health
ICAM1	0.00 ± 0.21	2.23 ± 1.00	0.85 ± 0.70	1.62 ± 0.37	TG, *	− 0.07 [− 0.14, 0.17]	1.90 [1.55, 3.07]	0.35 [0.09, 0.41]	3.29 [1.33, 3.50]	2 > 1, 4 > 1
OCLN	0.00 ± 0.19	0.14 ± 0.20	0.33 ± 0.18	0.19 ± 0.11	CCI	0.00 ± 0.17	0.08 ± 0.32	0.04 ± 0.16	0.00 ± 0.22	ns
TJP1	0.00 ± 0.12	− 0.13 ± 0.17	0.15 ± 0.18	0.05 ± 0.08	CCI	0.00 ± 0.04	0.00 ± 0.12	0.12 ± 0.10	− 0.02 ± 0.19	ns
VEGFA	− 0.01 [− 0.08, 0.09]	− 0.49 [− 0.56, − 0.09]	0.18 [− 0.24, 0.93]	− 0.26 [− 0.51, − 0.11]	ns	0.00 ± 0.08	− 0.26 ± 0.09	− 0.06 ± 0.30	− 0.48 ± 0.26	TG
SYNAPTOPHYSIN	0.05 [− 0.13, 0.11]	− 0.21 [− 0.46, − 0.09]	− 0.03 [− 0.10, 0.06]	0.00 [− 0.11, 0.14]	ns	0.00 ± 0.18	− 0.07 ± 0.22	0.01 ± 0.11	− 0.28 ± 0.19	TG
HIF1A	0.00 ± 0.16	− 0.18 ± 0.28	− 0.08 ± 0.14	0.06 ± 0.20	ns	0.00 ± 0.17	0.12 ± 0.13	0.07 ± 0.12	0.21 ± 0.32	ns
MAP2	0.00 ± 0.09	− 0.27 ± 0.28	− 0.09 ± 0.23	− 0.08 ± 0.03	TG, CCI	0.00 ± 0.11	− 0.03 ± 0.06	0.01 ± 0.14	− 0.35 ± 0.12	TG, CCI, *

Normally distributed data were analyzed with a 2-way ANOVA and are presented as mean ± SD. Under the ‘Significance’ column, ‘TG’ indicates a significant ANOVA main effect for T. gondii, ‘CCI’ indicates a significant ANOVA main effect for CCI, and ‘*’ indicates a significant ANOVA interaction between T. gondii and CCI. Post-hoc findings of interactive effects are detailed in the results section and Fig. 3. Non-normally distributed data were analyzed with Kruskal–Wallis tests and are presented as median [25,75 percentile]. Under the ‘Significance’ column, group differences identified with Bonferroni-corrected comparisons are summarized using the following group labels: 1 = Vehicle + Sham, 2 = T. gondii + Sham, 3 = Vehicle + CCI, 4 = T. gondii + CCI. ns = not statistically significant (i.e., p > 0.05)

ISSN: 1742-2094