Fig. 4

ATF3-positive neurons are labeled by GFP in Atf3:BAC Tg mice after RCS loading. a Atf3:BAC Tg mouse labels mitochondria with GFP upon the expression of ATF3. b–g GFP and ATF3 expression in DRG neurons of control (CTR) (b–d) and RCS (e–g) mice at 7 days of RCS loading. The white dotted lines indicate the edge of the DRG. h High-power magnification photographs clearly demonstrate that cell nuclei were stained by ATF3 (red) (arrowheads), whereas the cytoplasm was labeled with GFP (green). i, j Percentages of ATF3- (i) or GFP-positive (j) neurons, as a percentage of DAPI-positive DRG neurons. n = 5 mice, *p < 0.0001. Error bars indicate the SEM. Unpaired t-test. k Whole-mount images showing that GFP-tagged mitochondria (mtGFP) were present in the central branch of the dorsal root from L3 to S1. Scale bars, 200 μm in b–g, 20 μm in h, k