Fig. 5

GFP-positive neurons in the VH of Atf3:BAC Tg mice after RCS are motor neurons. a, b GFP expression in the VH of the spinal cord of Atf3:BAC Tg mouse before (Atf3:BAC CTR) (a) and after 7 days of RCS (Atf3:BAC RCS) (b). c–f GFP expression (green) in the dorsal region of VH of the spinal cord in ATF3- (red) and ChAT-positive (light blue) motor neurons. g, h Graph showing the quantification of ATF3- or GFP-positive neurons, as a percentage of ChAT-positive neurons. n = 4 mice for CTR, n = 5 mice for RCS group. *p < 0.0001. Error bars indicate the SEM. Unpaired t-test. i Whole-mount images showing that GFP-tagged mitochondria (mtGFP) were present in the ventral root from L3 to S1. The white dotted lines indicate the edge of spinal cord in a–f. Scale bars, 200 μm in a, b, 100 μm in c–f and 20 μm in i