Fig. 7

GFP-positive fiber trajectory in Atf3:BAC Tg mice after RCS exposure. a, b GFP expression in Atf3:BAC Tg mice before (a) and after (b) 7 days of RCS. Note that GFP expression can be observed from the medial part of the nucleus proprius (deep layers of the DH) to the motor neurons located in the dorsal part of the VH. The boxed area in b is magnified in c. d–f Injection of the neural tracer CTXb into the IMF of RCS-loaded Atf3:BAC Tg mice revealed the co-expression of GFP and CTXb in motor neurons and their fibers (arrowheads). GFP-positive fibers in the circled area may be central branch terminals from proprioceptive DRG neurons. The white dotted lines indicate the edge of spinal cord. Scale bars, 200 μm in a, b, 50 μm in c, 100 μm in d–f