Fig. 9

Microglia accumulate along the trajectory of the GFP-positive reflex arc in Atf3:BAC Tg mice. a, b Low-power magnification of Iba1 and GFP expression in the spinal cord of control (CTR) Atf3:BAC Tg mouse. Areas 1–3, surrounded by dotted boxes, indicate the areas for the images in c–e for Iba1 and f–h for GFP. i, j Low-power magnification of Iba1 and GFP expression in the spinal cord of RCS-loaded Atf3:BAC Tg mouse. Areas 1–3, surrounded by dotted boxes, indicate the areas for the images in k–m for Iba1 and n–p for GFP. q Quantification of the number of Iba1-positive cells in areas 1–3, shown in a, i. r GFP-positive area in areas 1–3, shown in b, j. n = 5 mice, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. Error bars indicate the SEM. Unpaired t-test. Scale bars, 100 μm in a, b, i, j and 50 μm in c–h, k–p