Fig. 4

EXOs-LPS-derived miR-615-5p can target MYRF 3′UTR. A Schematic diagram of targeted binding sequence between miR-615-5p and MYRF 3′UTR. B Luciferase activity was tested by the one-GLo™ EX Luciferase Assay System in 293 T cells. C After OPCs transfection with miR-615-5p mimics, the expressions of MYRF and β-actin were detected by qRT-PCR. D After OPCs transfection with miR-615-5p mimics, the expressions of MYRF and β-actin were detected by WB, and E is the quantification of MYRF by Image J software. F CNPase and Olig2 were immunostained for the OPCs transfected with miR-615-5p mimics. The magnification of cells is shown on the right. G The percentage of CNPase⁺Olig2⁺ cell numbers divided by the total number of cells. H The relative percentage of three differentiation stages of OPCs in each experimental group. I Immunofluorescence staining to detect the expression of CNPase, and J the percentage of CNPase⁺ cells. Each data point represents the average of at least 4-6 regions of interest analyzed in each well, n ≥ 3 wells per group. All data are represented by mean ± SEM. T-tests were used to determine P values (C, E). One-way ANOVA was used to determine P values (B, G, J). *P < 0.05, **P < 0.01, ***P < 0.001. Two-way ANOVA was used to determine P values (H). One representative of three independent experiments is shown. Scale bar = 100 μm (F, I)