Fig. 6

Antagonizing miR-615-5p can alleviate demyelination in EAE mice. A Immunolabeling of PDGFRα and APC in the spinal cords, and B, C the number of PDGFRα⁺ and APC⁺ cells per mm². D TEM images of the spinal cords in different experimental groups. Magnified views of partial myelin sheaths are shown below. Red represents regenerated myelin, yellow represents demyelinated sheath, and blue represents normal myelin. E Number of remyelinated axons. F Percentage of myelinated axons to total axons. G Scatter plot of myelin sheath thickness. H Quantification of the G-ratios (axon diameter/fiber diameter) of myelinated fibers. I, J The expression of MYRF and MBP mRNA was detected by qRT-PCR in the brain and spinal cords. K After antagonizing miR-615-5p, the expressions of MYRF, MBP, and β-actin in the spinal cords were detected by WB. L, M Image J software quantification statistical results. Each data point represents the average of 4–6 regions of interest analyzed per section from at least 3 sections per animal with n ≥ 3 mice per group. Spinal cord samples analyzed in the white matter lesion area (A, D). Typical representative figures show the dorsal cord lesion are of the spinal cord(A). All data are represented by mean ± SEM. One-way ANOVA was used to determine P values (B, C, E–H, L, M). Two-way ANOVA was used to determine P values (I, J). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. One representative of three independent experiments is shown. Scale bar = 100 μm (A) and 2 μm (D)