Fig. 7

Antagonizing miR-615-5p in microglia significantly alleviated demyelination in the CPZ model. A Mice were divided into four groups: Naïve group, CPZ group, AAV-Scramble group, and AAV-miR-615-5p-Sponge group. One week after feeding 0.2% CPZ diet, AAVs were injected into mice via the lateral ventricles. After 5 weeks of feeding 0.2% CPZ diet, the behavioral test was performed, and brains were harvested for black gold and IF testing. B, C The motor coordinative function was assessed by tight rope test and fatigue baton twister test. D Immunofluorescence staining of Iba1 and GFP in each group is shown, and E the number of Iba1⁺GFP⁺ cells per mm². F Black gold staining of myelinated axons and G Black gold myelin per area. H TEM images of the brains in different experimental groups. I Percentage of myelinated axons to total axons. J Quantifying the G-ratios (axon diameter/fiber diameter) of myelinated fibers. K Scatter plots of myelin sheath thickness. L Immunolabeling of PDGFRα and APC in the Brains, and M, N the number of PDGFRα⁺ and APC⁺ cells per mm². O Immunolabeling of MYRF and SOX10 in the Brains, and P the number of MYRF⁺ SOX10⁺ cells per mm². Each data point represents the average of 4–6 regions of interest analyzed per section from at least 3 sections per animal with n ≥ 3 mice per group. Sites of lesions in the CC region analyzed in mouse brain samples. One-way ANOVA was used to determine P values (B, C, E, G, I, J, K, M, N, P). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. One representative of three independent experiments is shown. Scale bar = 50 μm (D, L, O) and 5 μm (F)